Bemerton’s FA Cup journey came to an end for this season, going down 1-0 to Southern League opponents Sholing.
The hosts, once again reduced to ten-men, lost George Bennett when he was shown a red as Harlequins were chasing for an equaliser.
To make matters worse, for the second successive competitive match, Ian Saunders’s men were denied a blatant penalty.
During the opening stages of the match, both teams had half chances, the first effort of note was when Claudio Herbert won a free kick for Sholing on the edge of the box.
He took the set-piece and his low shot was saved well by Creese to his bottom right corner.
The next big moment was the penalty incident. The industrious Marvin Mclean pulled off a block that both sides keepers would have been proud of, punching the ball over the bar from a dangerous volley.
Both referee and assistant gave a corner, much to the frustration of the Harlequins players and officials.
By half time, a video of the incident was being shown on the terraces and subsequently shared on social media, detailing just how blatant the incident was.
With the sides in deadlock at the break, it was Harlequins who threatened first in the second half.
A fantastic corner from Danny Young found skipper Jack Smith at the far post, who headed over the top of the goal.
That miss would prove crucial, as minutes later Sholing drew first blood, Dan Mason played in Stuart Green who slotted the ball home, past a helpless Creese.
Green won promotion to the Southern Premier League with neighbours Salisbury in 2018 and shone in front of many of his former teammates.
Five minutes later Claudio Herbert nearly doubled the lead for Sholing when his powerful shot hit the bar, but the ball landed safely, the right side of the line.
The match came to a stop for quite some time when Charlie Burden fell after a sickening accidental aerial clash.
Both sides medical teams quickly ran to the young midfielders aid, before he left the action on a stretcher.
Justin Bennett went closest to drawing level as Harlequins, but the task was made tougher when George Bennett saw red after a foul on Stuart Green.
He was in fact dismissed for dissent, after protesting the decision with the referee.
Herbert once again struck the hosts crossbar before the action was over.
By Thom Belk